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为探讨山莨菪碱 (ADM )防治内毒素性急性肺损伤 (ALI)的效果及其分子机制 ,采用动物随机分成正常对照组 (NS) ,内毒素损伤组 (LPS)和山莨菪碱组 (ADM ) ,分别观察肺泡灌洗液 (BALF)的WBC分类及计数 ,血浆及BALF中TNF浓度 ,P6 5表达。结果显示血浆肿瘤坏死因子 (TNF)和BALF浓度变化在注射LPS后各时点 ,ADM组与LPS组相比较 ,TNF浓度增高明显减少 (P <0 0 1) ;注射LPS 5h后NS组和ADM组肺组织未见P6 5表达 ,而LPS组则可见P6 5表达。说明ADM可明显减轻LPS导致的ALI,ADM抑制P6 5表达可能是其抗肺损伤的重要机制之一
To investigate the effect and mechanism of anisodamine on the prevention and treatment of endotoxin-induced acute lung injury (ALI) in rats, the animals were randomly divided into normal control group (NS), endotoxin injury group (LPS) and anisodamine group ). The WBC classification and counting of bronchoalveolar lavage fluid (BALF), the concentration of TNF in plasma and BALF, and the expression of P6 5 were observed. The results showed that plasma TNF and BALF concentrations at different time points after LPS injection were significantly lower than those in LPS group (P <0.01) No lung tissue P6 5 expression, while LPS group can be seen P6 5 expression. ADM can significantly reduce the ALI caused by LPS, ADM inhibition of P6 5 expression may be one of the important mechanisms of anti-lung injury