论文部分内容阅读
已有研究表明,局部应用碱性成纤维细胞生长因子(bFGF或FGF-2)可促进实验动物牙周损伤的再生修复。本实验对牙周膜(PDL)细胞表面FGF受体表达情况作研究,进一步探讨FGF-2对PDL细胞作用的生物学机制。 材料和方法 取正畸拔除的健康第一前磨牙根中部牙周膜组织,采用组织块培养法,建立人PDL细胞系,第4～5代用于以下实验:①荧光法测定PDL细胞标准培养23 d中DNA含量变化。②以p-NP为底物测定PDL细胞生长过程中不同时间碱性磷酸酶(ALP)活性,并计算每微克DNA所对应的ALP活性单位。③测定在加入FGF-2条件下不同时间ALP活性,并与无
Studies have shown that local application of basic fibroblast growth factor (bFGF or FGF-2) can promote the regeneration of periodontal injury in experimental animals. In this study, we investigated the expression of FGF receptor on the surface of PDL cells and further explored the biological mechanism of FGF-2 on PDL cells. Materials and Methods The human PDL cell line was established by tissue culture method in the periodontal ligament tissue of the root of the first premolar tooth from orthodontic extraction. The 4th to 5th generations were used in the following experiments: ①Determination of PDL cells by fluorescence method 23 d DNA content changes. ② The activity of alkaline phosphatase (ALP) at different time during the growth of PDL cells was measured by using p-NP as a substrate, and the ALP activity unit corresponding to each microgram of DNA was calculated. ③ Determination of the addition of FGF-2 at different times ALP activity, and with no