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作者合成了一种含有免疫刺激剂和结合人IL-1β序列的IL-1受体的肽,称之为“bonsai”的细胞介素-1(bIL-1),并试验其免疫佐剂性质。将bIL-1加入血源性乙型肝炎疫苗中,免疫BALB/c小鼠,基础免疫后14天采血,在21天时加强免疫,在28天再釆血,应用ELISA法检测血清中抗-HBs水平。结果:用加bIL-1的疫苗基础免疫后,抗-HBs的IgM和IgG抗体均升高;加强免疫后,抗-HBs的IgG抗体比单独用疫苗高2倍。抗-“a”抗体比单独用疫苗高5～7倍。应用加bIL-1的疫苗免疫C5781/6小
The authors synthesized a peptide that contains an immunostimulant and an IL-1 receptor that binds to the human IL-1β sequence, and called it “bonsai”, a cytokine-1 (bIL-1), and tested its immunological adjuvant properties . BALB / c mice were immunized by adding bIL-1 into blood-borne hepatitis B vaccine. Blood was collected 14 days after the basic immunization, and boosted at 21 days. Blood samples were collected at 28 days. Serum anti-HBs Level. RESULTS: IgM and IgG antibodies to anti-HBs were elevated after basal immunization with bIL-1; IgG antibodies to anti-HBs were 2-fold more potent than vaccination alone after booster immunization. Anti-“a” antibody is 5 to 7 times higher than the vaccine alone. C5781 / 6 mice were immunized with the vaccine plus bIL-1