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研究致肾盂肾炎大肠杆菌Ｐ菌毛粘附素血清型，为该菌的临床鉴定奠定基础。方法：利用６个带有致肾盂肾炎大肠杆菌不同血清型Ｐ菌毛粘附素基因群的克隆株免疫新西兰白兔获得抗血清，经吸收后仅保留对各血清型粘附基因群的特异性。采用单价和多价抗血清建立了全菌ＥＬＩＳＡ方法，用于９５株尿源性大肠杆菌的研究，并与血凝试验进行比较。结果：９５株受检菌株中，ＭＲＨＡ阳性菌株中３７．３％（２５／６７株）全菌ＥＬＩＳＡ阳性，其中ＭＲＨＡ＋＋＋～＋＋＋＋菌株占 ８８．０％（２２／２５）株，ＭＲＨＡ阴性菌株中全菌ＥＬＩＳＡ阳性者仅为 ３．６％（１／２８）株。２６株全菌ＥＬＩＳＡ阳性菌Ｐ菌毛粘附素，Ｆ１３型为８８．５％，Ｆ７型为１１．５％。结论：采用Ｆ１３型或多价抗血清建立的全菌ＥＬＩＳＡ方法可用于ＵＰＥＣ的鉴定。
Study of pyelonephritis caused by pyelonephritis P pneumonia adhesin serotype, to lay the foundation for the clinical identification of the bacteria. Methods: Six New Zealand white rabbits were immunized with cloned strains of different P pneumoniae adhesin gene of Escherichia coli causing pyelonephritis. The antisera were obtained after absorption and retained only the specificity of each serotype adhesion gene. An all-bacteria ELISA was developed using monovalent and multivalent antisera for 95 urinary Escherichia coli strains and compared with the clotting test. Results: Of the 95 tested strains, 37.3% (25/67) of MRHA positive strains were positive for whole bacteria ELISA, of which MRHA +++ ~ ++++ strains accounted for 88.0% (22/25), MRHA negative strains Only 4.0% (1/28) strains of bacteria were positive for the ELISA. 26 strains of Pseudomonas aeruginosa positive bacteria P pilus adhesin, F13 type was 88.5%, F7 type was 11.5%. Conclusion: The whole-bacteria ELISA method with F13 or multi-valent antiserum can be used for the identification of UPEC.