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目的通过建立非外源性诱导物引发的实验性外伤性视网膜脱离动物模型,探讨外伤性视网膜脱离后视网膜的组织学改变。方法兔眼后上方角膜缘后2 mm平行切开巩膜,挤压出玻璃体,并向神经上皮层下注入眼用BSS,造成视网膜180°脱离,8-0可吸收线缝合伤口。以此制造外伤性视网膜脱离的动物模型。组织病理学光镜及电镜观察视网膜脱离模型视网膜的病理学变化。结果视网膜脱离后视网膜各层出现不同程度的退行性改变,以光感受器受损最为显著,神经元细胞成分减少、结构破坏,Müller细胞出现增生、肥大及胶质化等改变。结论此模型视网膜脱离发生率高,后期有PVR的发生,可用于视网膜脱离及引发增殖性玻璃体视网膜病变的研究。
OBJECTIVE: To establish an animal model of experimental traumatic retinal detachment induced by non-exogenous inducers and to investigate the histological changes of the retina after traumatic retinal detachment. Methods The sclera was cut parallelly 2 mm posterior to the corneal limbus. The vitreous body was squeezed out and the BSS was injected into the neuroepithelial layer. The retina was detached by 180 ° and the suture was wound by 8-0 absorbable suture. As a result, an animal model of traumatic retinal detachment was made. Histopathological light microscope and electron microscope were used to observe the pathological changes of retina in retinal detachment model. Results Retinal detachment showed varying degrees of degenerative changes in the retina to photoreceptor damage the most significant reduction in neuronal cell components, structural damage, Müller cells appear hyperplasia, hypertrophy and glia and other changes. Conclusion The incidence of retinal detachment in this model is high, and the occurrence of PVR in later stage can be used in the study of retinal detachment and priming of proliferative vitreoretinopathy.