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以往我们采用冷藏法保藏菌种,从种菇收集到的担孢子须立即分离,获得一级纯母种又须置于冰箱(4～10℃)保藏2～3个月,再经二次传代,制成三级生产菌种。这种方法具有防止菌种传代多而老化衰退,保持菌种纯壮的作用,但它仅能保藏菌丝体(不能很好地保藏有性担孢子),且菌丝体久置冰箱极易感染杂菌,以及置于冰箱的菌丝
In the past we used cold preservation of bacteria, collected from the species of the spores must be immediately separated to obtain a pure mother and must be placed in the refrigerator (4 ~ 10 ℃) for 2 to 3 months, and then by the second passage , Made of three production strains. This method has the function of preventing the passage of bacteria from aging and deteriorating, and maintains the pure and strong fungus. However, it can only preserve the mycelium (can not well preserve the sexual basidiomycete), and the mycelium is easy to store in the refrigerator for a long time. Infected with bacteria, as well as placed in the refrigerator hyphae