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本文描述了用二阶导数荧光光度法同时测定色氨酸和酪氨酸。在 p H 7.4的条件下 ,用 2 2 1nm作为激发波长 ,记录色氨酸和酪氨酸的发射光谱 ,并进行二阶导数处理。色氨酸在 318nm处 ,酪氨酸在 2 83nm处 ,二阶导数峰高与浓度成线性关系。色氨酸工作曲线的线性回归方程为 c =0 .0 0 0 7H - 0 .0 0 4,r =0 .996 4,线性范围为 0 .0 0 4到 0 .2 0 0μg . m L- 1 。酪氨酸工作曲线的线性回归方程为 c =0 .0 0 12 H -0 .0 0 40 ,r=0 .9971。线性范围为 0 .0 0 2到 0 .2 5 0 μg· m L- 1 。实验了 p H、温度和干扰离子对测定的影响 ,测定了苹果中的色氨酸和酪氨酸的含量 ,回收率分别为 (92 .0～ 10 4.0 ) %和 (98.70～ 10 2 .0 ) % ,相对标准偏差分别为 3.5 %和 2 .8%。
This paper describes the simultaneous determination of tryptophan and tyrosine by second derivative fluorescence spectroscopy. The emission spectra of tryptophan and tyrosine were recorded under the condition of p H 7.4 using 2 2 1 nm as the excitation wavelength and subjected to the second derivative treatment. Tryptophan at 318nm, tyrosine at 2383nm, the second derivative peak height and concentration of a linear relationship. The linear regression equation for the tryptophan working curve is c = 0.00007H -0.040, r = 0.996 4, and the linear range is 0.040 to 0.200 μg m L- 1 . The linear regression equation of tyrosine working curve is c = 0. 0 12 H -0 .0 0 40, r = 0 .9971. The linear range was from 0.002 to 0.250 μg · mL -1. The effects of p H, temperature and interfering ions on the determination were tested. The content of tryptophan and tyrosine in apple was determined. The recoveries were (92.0 ~ 10 4.0)% and (98.70 ~ 102.0 )%, The relative standard deviations were 3.5% and 2.8% respectively.