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以高抗青枯病的茄子高代自交系‘Q31-1’为试材,采用同源重组法构建了感染青枯病原菌R.solanacearum的茄子根部酵母双杂交cDNA文库,以期为研究茄子(Solanum melongena L.)与青枯病病原菌R.solanacearum的互作机制奠定基础。结果表明:文库库容大于1.25×10~7 CFU,平均插入片段大于1.5kb,随机挑选24个单克隆进行PCR检测,阳性率为100%。表明该文库可用于进行下一步互作蛋白筛选的研究。
In order to study the effect of solanum melongena (Solanum melongena) on the inbred line ’Q31-1’ with high resistance to bacterial wilt, a yeast two-hybrid cDNA library of R. solanacearum infected with R. solanacearum was constructed by homologous recombination. L.) and the bacterial wilt pathogen R. solanacearum lay the foundation for the interaction mechanism. The results showed that the library capacity was greater than 1.25 × 10 ~ 7 CFU, with an average insert size greater than 1.5kb. 24 clones were randomly selected for PCR detection. The positive rate was 100%. Indicating that the library can be used for the next step of the interaction protein screening research.