论文部分内容阅读
为探讨三氧化二砷 (As2 O3)对肝癌HepG2细胞的增殖抑制作用 ,应用台盼蓝拒染法、MTT比色法及细胞克隆形成试验 ,研究As2 O3 对肝癌细胞的增殖抑制和细胞毒作用。结果发现As2 O3 能显著抑制肝癌细胞的生长 ,5 μmol/LAs2 O3 抑制程度明显强于 1μmol/L。 5 μmol/L和 1μmol/LAs2 O3 作用后细胞克隆形成率分别为 1.5± 1.2 %和 12 3± 2 .2 % ,明显低于对照组的 30 .0± 4.2 % (P <0 .0 1)。As2 O3 对肝癌细胞有较强的细胞毒作用 ,且呈显著剂量和时间依赖性。其对于肝癌细胞的杀伤率高于 5 氟脲嘧啶 ( 5 FU )和丝裂霉素 (MMC) ,但无显著差异。As2 O3 与 5 FU及MMC存在协同效应 ,联合应用 ,对肝癌细胞的杀伤率明显升高。说明As2 O3 具有明显的抑制肝癌细胞增殖的作用 ,有必要进一步探讨其对肝癌的治疗价值
To investigate the inhibitory effect of arsenic trioxide (As2O3) on hepatocellular carcinoma HepG2 cells, trypan blue exclusion assay, MTT colorimetric assay and cell clone formation assay were used to study the inhibitory and cytotoxic effects of As2O3 on hepatoma cells. The results showed that As2O3 can significantly inhibit the growth of hepatoma cells, and the inhibition of 5 μmol/LAs2 O3 was significantly stronger than that of 1 μmol/L. The colony formation rate after 5 μmol/L and 1 μmol/L azO3 treatment was 1.5± 1.2 % and 12 3± 2 .2 %, respectively, which was significantly lower than 30. 0± 4.2 % in the control group (P <0 .0 1). . As2O3 has a strong cytotoxic effect on hepatoma cells in a dose- and time-dependent manner. The killing rate for hepatoma cells was higher than 5 fluorouracil (5 FU) and mitomycin (MMC), but there was no significant difference. As2O3 had synergistic effects with 5 FU and MMC, and the combined use of these drugs significantly increased the killing rate of hepatoma cells. This shows that As2O3 has a significant inhibitory effect on the proliferation of hepatoma cells, and it is necessary to further explore its therapeutic value for liver cancer.