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[Objective] Study on the phylogenetic analysis of the 16S rDNA and insecticidal characteristics of strain BH-1 isolated from diseased larva of Anoplophora glabripennis(Motsch.) [Method] The strain was identified by routine method and inoculated onto healthy Anoplophora glabripennis(Motsch.) for observing insecticidal effect,further 16S DNA was amplified by the specific primers for sequencing and homology analysis. [Result] The mortality of second instar of Anoplophora glabripennis(Motsch.) reached 72.7% 8 d after 1010 cfu/ml BH-1 was inoculated. The homology of 16S DNA sequences between BH-1 and Serratia marcescens accessed in GenBank reached 99.5%. Combined with the results of routine identification,BH-1 was identified as S. marcescens. [Conclusion] BH-1 could be used for biological control of Anoplophora glabripennis (Motsch.).
[Objective] Study on the phylogenetic analysis of the 16S rDNA and insecticidal characteristics of strain BH-1 isolated from diseased larva of Anoplophora glabripennis (Motsch.) [Method] The strain was identified by routine method and inoculated onto healthy Anoplophora glabripennis (Motsch. ) for observing insecticidal effect, further 16S DNA was amplified by the specific primers for sequencing and homology analysis. [Result] The mortality of second instar of Anoplophora glabripennis (Motsch.) reached 72.7% 8 d after 1010 cfu / ml BH-1 was The homology of 16S DNA sequences between BH-1 and Serratia marcescens accessed in GenBank reached 99.5%. Combined with the results of routine identification, BH-1 was identified as S. marcescens. [Conclusion] BH-1 could be used for biological control of Anoplophora glabripennis (Motsch.).