The mechanisms for the effects of ammonium-based fertilizers on soil acidification in subtropical regions are not well understood.Two Ultisols collected from cropland and a tea garden in Anhui and Jiangxi Provinces in subtropical southern China,respectively,were used to study the effects of urea and(NH 4) 2 SO 4 on the nitrification and acidification of soils with incubation experiments.Nitrification occurred at very low pH with no N fertilizer added and led to lowering of the soil pH by 0.53 and 0.30 units for the soils from Jiangxi and Anhui,respectively.Addition of urea accelerated nitrification and soil acidification in both Ultisols;while nitrification was inhibited by the addition of(NH 4) 2 SO 4,and greater input of(NH 4) 2 SO 4 led to greater inhibition of nitrification.Ammonia-oxidizing bacteria(AOB) played an important role in nitrification in cropland soil under acidic conditions.Addition of urea increased the soil pH at the early stages of incubation due to hydrolysis and stimulated the increase in the AOB population,and thus accelerated nitrification and soil acidification.At the end of incubation,the pH of Ultisol from Jiangxi had decreased by 1.25,1.54 and 1.84 units compared to maximum values for the treatments with 150,300 and 400 mg/kg of urea-N added,respectively;the corresponding figures were 0.95,1.25 and 1.69 for the Ultisol from Anhui.However,addition of(NH 4) 2 SO 4 inhibited the increase in the AOB population and thus inhibited nitrification and soil acidification.Soil pH for the treatments with 300 and 400 mg/kg of(NH 4) 2 SO 4-N remained almost constant during the incubation.AOB played an important role in nitrification of the cropland soil under acidic conditions.Addition of urea stimulated the increase in the AOB population and thus accelerated nitrification and soil acidification;while addition of(NH 4) 2 SO 4 inhibited the increase in the AOB population and thus inhibited nitrification. The mechanisms for the effects of ammonium-based fertilizers on soil acidification in subtropical regions are not well understood. Two Ultisols collected from cropland and a tea garden in Anhui and Jiangxi Provinces in subtropical southern China, respectively, were used to study the effects of urea and (NH 4) 2 SO 4 on the nitrification and acidification of soils with incubation experiments. Nitrification occurred at very low pH with no N fertilizer added and led to lowering of the soil pH by 0.53 and 0.30 units for the soils from Jiangxi and Anhui , respectively. Addition of urea accelerated nitrification and soil acidification in both Ultisols; while nitrification was inhibited by addition of (NH 4) 2 SO 4, and greater input of (NH 4) 2 SO 4 led to greater inhibition of nitrification. Ammonia -oxidizing bacteria (AOB) played an important role in nitrification in cropland soil under acidic conditions. Addition of urea increased the soil pH at the early stages of incubation due to hydrolysis and s At the end of incubation, the pH of Ultisol from Jiangxi had decreased by 1.25, 1.54 and 1.84 units compared to maximum values ​​for the treatments with 150, 300 and 400 mg / kg of urea-N added, respectively; the corresponding figures were 0.95, 1.25 and 1.69 for the Ultisol from Anhui. Still, (of NH 4) 2 SO 4 inhibited the increase in the AOB population and thus inhibited nitrification and soil acidification. Soil pH for the treatments with 300 and 400 mg / kg of (NH 4) 2 SO 4-N an almost constant during the incubation. AOB played an important role in nitrification of the cropland soil under acidic conditions. Addition of urea stimulated the increase in the AOB population and thus accelerated nitrification and soil acidification; while addition of (NH 4) 2 SO 4 inhibited the increase in the AOB population and thus inhibited nitrification.