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混合唾液有大量含特异酶系统的微生物,微生物的酶对唾液的pH值有重要影响。把唾液的微生物分为两类,第一类含尿素酶,使尿素水解形成氨致唾液碱性化;第二类含糖酵解酶,使糖发酵产酸致唾液酸性化。口腔菌群正常时唾液的pH值为6.0～7.9或7.06～7.34。 为研究唾液微生物的类型,作者提出测定唾液的尿素水解活性和糖酵解活性的方法。收集空腹(或饭后2～3h)混合唾液进行检测,可被唾液分解的各种物质有尿素、葡萄糖、半乳糖、果糖、蔗糖、麦芽糖和乳糖;均配成0.1mol/L溶液5～10ml,冷藏防止空气中细菌污染繁殖。显示pH值的指示剂有酚红(pH6.4～8.0时为黄色至红色);溴麝香草脑蓝(pH6.2～7.6时为黄色至蓝色);溴甲酚紫(pH5.2～6.8时为黄色至紫色)。用80mm×65mm大小并有许多小槽的瓷板,瓷板温度为37℃,在小槽内放入唾液,尿素或各种糖类各0.1
Mixed saliva has a large number of specific enzyme-containing microorganisms, microbial enzymes have a significant impact on the pH of saliva. The saliva microorganisms are divided into two categories, the first category contains urease, ammonia hydrolysis to form ammonia to salivary alkaline; second glycolytic enzymes, the sugar fermentation acidification of sialic acid. Saliva pH value of oral flora normal 6.0 to 7.9 or 7.06 to 7.34. To investigate the types of salivary microbes, the authors propose a method to determine the saliva's urea-hydrolyzing activity and glycolysis activity. Collected fasting (or 2 ~ 3h after meal) mixed saliva for testing, can be saliva decomposition of various substances are urea, glucose, galactose, fructose, sucrose, maltose and lactose; are dubbed 0.1mol / L solution 5 ~ 10ml , Refrigerated to prevent bacteria in the air pollution breeding. Indicators showing pH were phenol red (yellow to red at pH 6.4 to 8.0); bromothymidine blue (yellow to blue at pH 6.2 to 7.6); bromocresol purple 6.8 when yellow to purple). With a size of 80mm × 65mm and many small tank porcelain, porcelain plate temperature of 37 ℃, in the small tank into the saliva, urea or various sugars 0.1