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2008年采用改进的F2代法和含毒饲料法监测河北省邱县和山东省无棣县田间棉铃虫(Helicoverpa armig-era)对Bt棉的抗性,结果表明:邱县和无棣县田间棉铃虫雌蛾普遍存在多次交配现象(1次交配率仅占30.0%~38.2%),改进的F2代法估测得这2个多次交配种群对Bt棉的抗性等位基因频率分别为0.082 6(0.031 1~0.134 0)和0.076 4(0.026 5~0.126 3);含毒饲料法测定这2个地区棉铃虫对Cry1Ac毒蛋白的抗性分别为20.9倍和12.5倍,达到中等水平抗性。说明2个地区棉铃虫的抗性已处于上升阶段,需尽快采取有效的抗性治理对策。
In 2008, the F2 generation method and the toxic feed method were used to monitor the resistance of Bt cotton to Helicoverpa armigera in Qiu County of Hebei Province and Wudi County in Shandong Province. The results showed that the resistance of Bt cotton to Qiu County and Wudi County The female moths of Helicoverpa armigera had many mating phenomena (only 30.0% ~ 38.2% of mating rate once), and the improved F2 generation method estimated the frequencies of the resistance alleles of these two multiple mating populations to Bt cotton Were 0.082 6 (0.031 1 ~ 0.134 0) and 0.076 4 (0.026 5 ~ 0.126 3). The resistance to Cry1Ac toxin in the two regions was 20.9 and 12.5 times higher than that of the control Resistant. The results showed that the resistance of H. armigera in two regions had been on the rising stage, and the effective resistance control strategy should be taken as soon as possible.