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采用化学发光法定量研究了高温、干旱、盐和重金属(镉和铜)等几种环境胁迫对大麦幼苗内源一氧化氮(NO)释放量的影响。结果表明:短期高温处理可诱导大麦幼苗内源NO释放上升,长期处理则降至对照水平;短期高浓度聚乙二醇6000(25%,30%)处理使植株NO释放量显著增加,但随干旱胁迫时间延长NO释放量有所下降;短期盐胁迫使植株NO释放量增加,长期处理则因盐度不同而有所差异,表现为中低盐度下(≤400mmol.L-1)植株NO释放与对照水平相当,而高盐度下(≥600mmol.L-1)植株NO释放则始终维持较高水平;短期重金属镉和铜处理植株后,植株NO释放与对照差异不大,长期镉处理后,植株NO释放显著升高,而长期铜处理只在低浓度下出现释放高峰。这些结果表明环境胁迫可诱导植物体内NO产生,其NO释放量与胁迫强度和作用时间有一定相关性。
The effects of environmental stress such as high temperature, drought, salt and heavy metals (cadmium and copper) on the release of endogenous nitric oxide (NO) from barley seedlings were quantitatively studied by chemiluminescence method. The results showed that the short-term high-temperature treatment could induce the increase of endogenous NO release in barley seedlings, while the long-term treatment decreased to the control level. Short-term high concentration of polyethylene glycol 6000 (25%, 30%) significantly increased NO release in plants, Short-term salt stress increased the amount of NO released by plants under long-term salinity stress, while salinity varied with long-term salinity stress, indicating that NO (≤400mmol.L-1) However, NO release in high-salinity (≥600mmol.L-1) plants maintained a high level at all times. After short-term heavy metal cadmium and copper treatment, there was no significant difference in NO release between the two plants and the long-term cadmium treatment After the treatment, the release of NO from plant significantly increased, while the long-term copper treatment showed the release peak only at low concentration. These results indicate that environmental stress can induce the production of NO in plants, and the amount of NO released correlates with the intensity of stress and the duration of action.