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目的：通过检测不孕不育症患者血清中抗缪勒氏管激素(anti-mul erian hormone,AMH),及卵泡刺激素(Fol icle-stimulating hormone,FSH)、黄体生成素(luteinizing hormone,LH)、雌二醇(Estradiol,E2)的血清表达水平,并进行比对、分析,探索AMH在不孕不育症中的临床应用价值。方法用化学发光法在ADVIA Centaur XP仪器上检测LH、FSH、E2,再用酶联免疫法对AMH进行测定,最后将这些指标用EXCEL软件进行线性回归分析。结果①根据厂商提供的AMH参考值可知,在FSH、LH、E2特别高的39例不孕不育育龄女性的标本中,38例都在该年龄段的参考范围内,只有1例超过参考范围,但是在不同年龄范围内低于中值年龄的30例患者中,有90.9%的患者AMH浓度远低于参考范围给出的中值浓度；②根据对LH、FSH、E2和AMH线性回归分析可知,LH、FSH、E2和AMH没有直接的线性关系；③LH/FSH呈一定程度的正相关。结论根据不同年龄段的育龄妇女的AMH浓度在该年龄段是否偏低或者异常增高来在一定程度上评估其生育能力,并通过进一步研究更为细分不同年龄段的妇女AMH表达情况,并建立合适的相应参考区间。 LH、FSH、E2和AMH没有太大的线性关系,这可能与LH、FSH、E2在月经周期期间波动很大、搜集的标本为不同年龄的、而AMH浓度不受月经周期影响、会受年龄影响有关。此外,有相关文章表明,经过年龄校正后LH/FSH的比值与AMH呈部分相关。而本试验做出LH/FSH呈一定程度的正相关,LH/FSH的比值是一个有用可靠评估卵巢储备功能的指标,并且可以和AMH共同应用于临床评估。因此AMH可以在一定程度应用于不孕不育症。“,”Objective Through the detection of anti-Mul erian hormone and fol icle stimulating hormone, luteinizing hormone, estradiol, comparison analysis, to explore the application value of AMH in the infertility.Methods The detection of LH, FSH, E2 in ADVIA Centaur XP on the instrument by direct chemiluminescence method, then ELISA method was used to determine AMH, final y the index regression analysis using EXCEL software linear.Results ①According to the manufacturer to provide the AMH reference values. It can be concluded that in the specimens of FSH, LH, E2 is particularly high for 39 cases of infertility in women of childbearing age, 38 cases are in the age of the reference range, only 1 case above the reference range, but in a dif erent age range below the median age of 30 patients, there are 90.9%of the patients with AMH concentrations far below reference range gives the median concentration. ② according to the regression analysis of LH, FSH, E2 and LH, FSH, AMH linear, E2 and AMH had no direct linear relationship. ③LH/FSH a certain degree of positive cor elation.Conclusion According to the kit in the reference range of AMH shows that, in 39 cases of infertility in women of childbearing age FSH, LH, E2 special high specimens, 38 cases were in the reference range of the age, only 1 cases of more than the reference range, but in dif erent age range is less than 30 of patients with a median age of the patients, there are with the concentration of AMH 90.9%is far below the median concentration reference range is given.Therefore, on the one hand, according to the concentration of AMH in dif erent age groups of women of childbearing age is low or abnormal to assess their fertility to a certain extent, on the other hand, the establishment of the reference range of AMH, can reduce the age range, in order to facilitate the screening of abnormal AMH in patients with a certain age. Also, according to the regression analysis of LH, FSH, E2 and AMH, FSH, E2 linear, LH and AMH had no linear relationship too big, which may be related to LH, FSH, E2 during the menstrual cycle fluctuated greatly, col ected specimens for dif erent age groups, but the concentration of AMH wil be influenced by the age . In addition, there are related articles that, after age adjusted odds ratio and AMH LH/FSH were related. This test is positively related to LH/FSH, the ratio of LH/FSH is a useful and reliable assessment of ovarian reserve function index, and can be applied to clinical evaluation and AMH. So AMH can be applied to infertility in a certain degree.According to the dif erent ages of women of childbearing age in the concentrations of AMH in the age is low or abnormal y increased to assess its ability to reproduce in a certain extent, and through further study of more segments of dif erent ages of women AMH expression, and the establishment of appropriate cor esponding reference intervals. LH, FSH, E2 and AMH is not too big a linear relationship, which may be related to LH, FSH, E2 during the menstrual cycle fluctuations and col ect specimens for dif erent age, and AMH concentrations from menstrual cycles, wil be af ected by age related. In addition, there are related articles that, after age adjusted odds ratio and AMH LH/FSH were related. And the test make LH/FSH showed a certain degree of positive cor elation, LH / FSH ratio is a useful and reliable evaluation of the ovarian reserve function index, and AMH and applied in clinical assessment. So AMH can be applied to infertility in a certain degree.