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对48例人肝细胞肝癌,肝硬变及20例正常肝组织的标本作丁免疫组化ABC法染色,观察了CerbB_(-2)蛋白的表达情况。发现CerbB_(-2)蛋白在正常肝组织,仅少数标本(2/20)中见到徽弱表达,而在肝细胞肝癌及其癌周肝硬变组织中均检测到CerbB_(-2),蛋白表达。阳性产物主要定位于肿瘤细胞及再生结节中的肝细胞膜和胞浆。这提示CerbB_(-2)基因的活化与人肝细胞肝癌发生有关,其作用机制可能促进肝硬变时肝细胞的增生向肝细胞不典型增生转化及促进不典型增生的进展。CerhB_(-2)阳性分级与肝细胞癌组织病理学分级呈显著性负相关(P<0.0005),组织学分级高,分化差的肝细胞癌,阳性级别低.可作为肝细胞癌的分化标志。
The specimens of 48 cases of human hepatocellular carcinoma, cirrhosis, and 20 normal liver tissues were stained with ABC to observe the expression of CerbB_(2) protein. CerbB_(-2) protein was found to be weakly expressed in only a few samples (2/20) in normal liver tissue, and CerbB_(-2) was detected in hepatocellular carcinoma and its surrounding cirrhosis. Protein. Positive products are mainly located in the hepatocyte membrane and cytoplasm of tumor cells and regenerating nodules. This suggests that the activation of CerbB_(-2) gene is related to the occurrence of human hepatocellular carcinoma, and its mechanism of action may promote the progression of hepatic hyperplasia to hepatocyte dysplasia and promote the progression of atypical hyperplasia. The positive grading of CerhB_(-2) was negatively correlated with the histopathological grade of hepatocellular carcinoma (P<0.0005). Histological grade was high, poorly differentiated hepatocellular carcinoma was of low positive grade and could be used as differentiation marker of hepatocellular carcinoma. .