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目的 探讨神经氨酸酶基因A(nanA)产物对感染肺炎球菌后仓鼠咽鼓管细胞表面糖化合物变化的作用。方法 应用植物血凝素组织化学技术 ,比较仓鼠在感染肺炎球菌 (D3 9菌株 )及其神经氨酸酶基因A缺陷株(ΔNA1)后咽鼓管细胞表面糖化合物的变化。结果 植物血凝素标记结果显示 ,D3 9感染组咽鼓管上皮细胞的LFA和SNA的染色比非感染对照组有明显下降 ,提示正常末端涎酸残基在D3 9组已被祛除。同时 ,咽鼓管腔表面WGA ,SuccWGA ,BSL II ,PNA和ECL在D3 9感染组的染色增强 ,而神经氨酸酶基因A缺陷株 (ΔNA1)感染组与非感染对照组相比 ,植物血凝素标记染色结果差异无显著性。结论 神经氨酸酶基因A(nanA)产物在改变细胞表面糖化合物结构的作用中起重要作用 ,由此导致肺炎球菌在仓鼠咽鼓管的种植和感染
Objective To investigate the effect of neuraminidase gene A (nanA) on the changes of carbohydrate compounds on the surface of eustachian tube cells in mice infected with pneumococcus. Methods Phytohemagglutinin histochemistry was used to compare the changes of carbohydrate compounds on the surface of eustachian tube cells in hamster infected with pneumococcus (D3 9 strain) and its neuraminidase gene defective (ΔNA1). Results The results of phytohemagglutinin labeling showed that the staining of LFA and SNA in eustachian tube epithelium of D3 9 infection group was significantly lower than that of non-infected control group, suggesting that the normal terminal sialic acid residues were dispelled in D3 9 group. At the same time, the staining of WGA, SuccWGA, BSL II, PNA and ECL in the pharyngeal cavity of the eustachian tube was enhanced in the D3 9 infection group. Compared with the non-infected control group, the neuraminidase gene A defective strain (ΔNA1) There was no significant difference in the results of lectin labeling staining. Conclusions The neuraminidase gene A (nanA) product plays an important role in altering the structure of sugar compounds on the cell surface, resulting in the construction and infection of pneumococci in the hamster’s eustachian tube