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目的:研究评价中药外用治疗骨转移癌疼痛的疗效和安全性。方法:实验使用雌性SD大鼠,随机分为假手术组、模型组、唑来膦酸组和中药组。假手术组大鼠左后肢胫骨的骨髓腔内注射Hank’s液,其余各组则在相同部位接种乳腺癌MRMT-1细胞以制成乳腺癌骨转移模型。次日开始对中药组用3%中药浸剂浸泡后肢。唑来膦酸组腹腔内注射唑来膦酸。模型组和假手术组浸泡后肢与去离子水,第21天取材,测定肿瘤体积,对胫骨标本胫骨标本用影像学方法进行评分。用免疫组化法对增殖细胞核抗原(PCNA)染色并进行半定量分析。结果:与模型组比较:中药组和唑来膦酸组影像学评分改善。中药组肿瘤体积减小(P<0.05),且与PCNA表达的下降趋势相一致。结论:外用中药可有效抑制骨转移肿瘤灶,减轻骨转移所致的溶骨性骨质破坏。
Objective: To evaluate the efficacy and safety of traditional Chinese medicine in the treatment of pain of bone metastases. Methods: Female SD rats were randomly divided into sham-operation group, model group, zoledronic acid group and Chinese medicine group. In the sham operation group, Hank’s solution was injected into the marrow of the tibia of the left hindlimb and the rest of the mice were inoculated with the MRMT-1 breast cancer cells in the same place to make the breast cancer bone metastasis model. The next day, the Chinese medicine group was immersed in hind limbs with 3% Chinese herbal infusion. Zoledronic acid was injected intraperitoneally into zoledronic acid. The hind limbs and deionized water were soaked in the model group and the sham operation group, and the tumor volume was measured on the 21st day. The tibial specimens of the tibia specimens were scored by the imaging method. Proliferating cell nuclear antigen (PCNA) was stained by immunohistochemistry and subjected to semi-quantitative analysis. Results: Compared with the model group, the imaging score of the traditional Chinese medicine group and zoledronic acid group improved. The tumor volume of the traditional Chinese medicine group decreased (P <0.05), and was consistent with the decreasing trend of PCNA expression. Conclusion: External use of traditional Chinese medicine can effectively inhibit the tumor metastasis of bone metastases, reduce osteolytic bone destruction caused by bone metastasis.