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为明确哈尔滨市及鸡西市稻瘟病菌致病性变化情况并优化鉴别体系,以24个水稻单基因系为鉴别体系,对2006—2008年采自2市的稻瘟病菌菌株进行致病型划分,并应用聚类分析方法完成鉴别体系优化。结果表明,应用水稻单基因系鉴别体系可将哈尔滨市2006—2008年采集的稻瘟病菌菌株划分为34、12和27个致病型,致病性相似系数分别为0.32~1.00、0~1.00和0.20~1.00;水稻单基因系对其抗性频率分别为2.86%~97.14%、8.33%~100.00%和10.00%~96.67%;鸡西市2006—2008年采集的菌株可划分为26、19和20个致病型,致病性相似系数分别为0.47~0.92、0.15~0.86和0.39~1.00;水稻单基因系对其抗性频率分别为3.85%~96.15%、5.26%~73.68%和4.76%~95.24%。适用于哈尔滨市的优化鉴别体系包括Pi-9、Pi-11、Pi-a、Pi-ks、Pi-5、Pi-i、Pi-sh、Pi-3、Pi-km和Pi-ta共10个基因,2006—2008年累计方差贡献率分别为86.15%、98.77%和87.39%;适用于鸡西市的优化鉴别体系包括Pi-9、Pi-11、Pi-a、Pi-ks、Pi-5、Pi-i、Pi-sh、Pi-1、Pi-7和Pi-t共10个基因,2006—2008年累计方差贡献率分别为82.58%、94.55%和90.37%。应用优化后的鉴别体系可将哈尔滨市2006—2008年采集的菌株划分为22、12和23个致病型,将鸡西市2006—2008年采集的菌株划分为22、14和18个致病型。
In order to clarify the pathogenicity of Magnaporthe grisea in Harbin and Jixi and to optimize the identification system, the pathogenicity of the two strains of Magnaporthe grisea in 2006-2008 were identified by using 24 single gene lines of rice as the identification system , And apply the cluster analysis method to complete the identification system optimization. The results showed that the strain of Magnaporthe grisea collected from 2006 to 2008 in Harbin could be divided into 34, 12 and 27 pathotypes using the single gene system for identification of rice. The similarity coefficients of pathogenicity were 0.32-1.00 and 0. 1.00 And 0.20-1.00 respectively. The resistance frequency of single-line rice was 2.86% -97.14%, 8.33% -100.00% and 10.00% -96.67%, respectively. The strains collected from 2006 to 2008 in Jixi were divided into 26, 19, The pathogenicity of 20 pathotypes were 0.47 ~ 0.92, 0.15 ~ 0.86 and 0.39 ~ 1.00, respectively. The resistance frequency of single-line rice was 3.85% ~ 96.15%, 5.26% ~ 73.68% and 4.76% ~ 95.24%. The optimal identification system for Harbin includes 10 for Pi-9, Pi-11, Pi-a, Pi-ks, Pi-5, Pi-i, Pi-sh, Pi-3, Pi-km and Pi-ta The results showed that the cumulative variance contribution rates in 2006-2008 were 86.15%, 98.77% and 87.39%, respectively. The optimal identification system for Jixi City included Pi-9, Pi-11, Pi-a, Pi-ks and Pi-5 , Pi-i, Pi-sh, Pi-1, Pi-7 and Pi-t. The cumulative variance contribution rates were 82.58%, 94.55% and 90.37% respectively from 2006 to 2008. After the application of the optimized identification system, the strains collected from 2006 to 2008 in Harbin can be divided into 22, 12 and 23 pathogenic types. The strains collected from 2006 to 2008 in Jixi were divided into 22, 14 and 18 pathogenic .