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目的：通过检测三七总皂甙（ＰＮＧＳ）对大鼠单离肝细胞Ｃａ２＋内流的影响，探讨其对肝缺血再灌注损伤保护作用的可能性。方法：采用胶原酶灌注法单离大鼠肝细胞，用４５Ｃａ示踪技术测定三七总皂甙对新鲜分离大鼠肝细胞Ｃａ２＋内流的影响。结果：肝细胞孵育在含４５Ｃａ生理液中，其细胞内４５Ｃａ浓度随孵育时间延长逐渐增高，１５ｍｉｎ时达高峰；２０ｎｍｏｌ·Ｌ－１垂体加压素使４５Ｃａ浓度增高３９％。Ｖｅｒａｐａｍｉｌ、Ｎｉｆｅｄｉｐｉｎｅ和ＰＮＧＳ均能不同程度地阻断静息状态和垂体加压素激动下的肝细胞４５Ｃａ内流，ＰＮＧＳ阻断效果明显优于Ｖｅｒａｐａｍｉｌ和Ｎｉｆｅｄｉｐｉｎｅ，且呈剂量依赖性。结论：ＰＮＧＳ具有特异性阻断肝细胞受体依赖性钙通道（ＲＯＣ）的作用，是一种理想的肝细胞钙通道阻滞剂。
OBJECTIVE: To investigate the effect of panax notoginseng saponins (PNGS) on Ca2+ influx in isolated hepatocytes of rats and explore the possibility of protective effects of hepatic ischemia-reperfusion injury. METHODS: Rat hepatocytes were isolated by collagenase perfusion method. The effects of panax notoginseng saponins on the Ca2+ influx of freshly isolated rat hepatocytes were determined by 45Ca tracer technique. Results: Incubation of hepatocytes in physiological fluid containing 45Ca, the intracellular concentration of 45Ca gradually increased with the incubation time, peaked at 15 min; 20nmol·L-1 pituitary vasopressin increased the concentration of 45Ca by 39%. Verapamil, Nifedipine and PNGS could block the resting state and 45Ca influx of hepatocytes under the action of pituitary vasopressin to varying degrees. The blocking effect of PNGS was significantly better than that of Verapamil and Nifedipine in a dose-dependent manner. Conclusion: PNGS has a specific role in blocking the receptor-dependent calcium channel (ROC) of hepatocytes and is an ideal calcium channel blocker for hepatocytes.