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用地高辛配基（Ｄｉｇ－）标记我国肾综合征出血热（ＨＦＲＳ）病毒Ｒ２２株Ｍ片段ｃＤＮＡＲ３克隆探针，对ＨＦＲＳ病毒Ｒ２２株感染的人血管内皮细胞（ＨＥＣｓ）作核酸原位杂交。杂交结果显示在受染的血管内皮细胞胞浆内有明显的杂交信号，而正常细胞和ＨＦＲＳ病毒感染细胞经ＲＮＡ酶处理组不出现杂交信号。结果表明ＨＦＲＳ病毒ＲＮＡ主要定位于血管内皮细胞的细胞浆中，提示病毒ＲＮＡ在胞浆中复制
Digoxin was used to label the cDNAR3 cloned probe of M segment of R22 strain of HFRS virus in China. The in situ hybridization was performed on human vascular endothelial cells (HECs) infected with HFRS virus R22 strain. Hybridization results showed that there was obvious hybridization signal in the cytoplasm of infected vascular endothelial cells, while no signal was observed in normal cells and HFRS virus infected cells. The results showed that HFRS virus RNA mainly localized in the cytoplasm of vascular endothelial cells, suggesting that viral RNA in the cytoplasm of replication