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目的：观察外源性超氧化物歧化酶（ｓｕｐｅｒｏｘｉｄｅｄｉｓｍｕｔａｓｅ，ＳＯＤ）和过氧化氢酶（ｃａｔａｌａｓｅ，ＣＡＴ）对培养的人视网膜色素上皮（ｒｅｔｉｎａｌｐｉｇｍｅｎｔｅｐｉｔｈｅｌｉｕｍ，ＲＰＥ）细胞光化学损伤的保护作用。方法：将铺满期原代培养的人ＲＰＥ细胞分为三组。Ａ组直接作光照处理，Ｂ组在培养液中加入ＳＯＤ（９０Ｕ／ｍｌ）和ＣＡＴ（９００Ｕ／ｍｌ）后３０分钟进行光照，Ｃ组为对照组。光照强度２４００Ｌｘ，时间６小时。处理结束后将三组细胞以２．４×１０５／ｍｌ的密度分别接种于２４孔板，每孔０．４ｍｌ。于接种后１、３、５和７天观察细胞形态，并进行细胞计数。结果：光照后３天时，Ａ、Ｂ组细胞生长缓慢，Ｃ组细胞生长活跃；３天后Ｂ组细胞生长接近Ｃ组，比Ａ组明显活跃（Ｐ＜０．０５）。结论：可见光可使培养的人ＲＰＥ细胞生长能力降低，外源性ＳＯＤ和ＣＡＴ对细胞的损伤具有保护作用。
Objective: To observe the protective effects of exogenous superoxidedismutase (SOD) and catalase (CAT) on the photochemical damage of cultured human retinal pigment epithelium (RPE) cells. Methods: The primary cultured human RPE cells were divided into three groups. Group A was exposed to light immediately. Group B was exposed to light (90 U / ml) and CAT (900 U / ml) for 30 minutes in group B, and control group. Light intensity 2400Lx, time 6 hours. After the treatment, three groups of cells were inoculated into 24-well plates at a density of 2.4 × 10 5 / ml, respectively, at a volume of 0.4 ml per well. Cell morphology was observed 1, 3, 5 and 7 days after inoculation and cell counts were performed. Results: After 3 days, the cells in group A and B grew slowly and the cells in group C grew actively. After 3 days, the cell growth in group B was close to that in group C, which was significantly more active than that in group A (P <0.05). CONCLUSION: Visible light can reduce the growth ability of cultured human RPE cells, and exogenous SOD and CAT have a protective effect on cell injury.