A novel extractant, N, N’-diacylpiperazine (DAPEZ), was synthesized and characterized for the first time. Its extraction property for U(VI) from aqueous nitric acid media has been studied. The effects of concentration of nitric acid and extractant on the distribution ratio were examined and the extraction mechanism were discussed. A novel extractant, N, N’-diacylpiperazine (DAPEZ), was synthesized and characterized for the first time. Its extraction property for U (VI) from aqueous nitric acid media has been studied. The effects of concentration of nitric acid and extractant on the distribution ratio were examined and the extraction mechanism were discussed.