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目的研究碱性成纤维细胞生长因子（ｂＦＧＦ）ｍＲＮＡ在正常、缺血以及缺血和再灌流骨骼肌中的表达。方法利用大鼠后肢缺血再灌流损伤模型，采用原位杂交方法与定量ＰＣＲ技术，观察ｂＦＧＦｍＲＮＡ在正常与创伤骨骼肌的定位特征与表达量的改变。结果在未缺血骨骼肌，ｂＦＧＦｍＲＮＡ染色为棕黑色，呈点状或点片状弥散分布于胞浆中，其阳性表达率为８２％。在缺血以及缺血与再灌流损伤的骨骼肌，ｂＦＧＦｍＲＮＡ的分布方式没有改变，但其染色强度与密度却明显减弱，肌纤维ｂＦＧＦｍＲＮＡ阳性染色率分别为５２％和２２％。结论创伤导致骨骼肌内源性ｂＦＧＦｍＲＮＡ表达量减少，进而导致ｂＦＧＦ产生减少，是缺血与再灌流条件下骨骼肌中内源性ｂＦＧＦ含量减少的重要原因之一。
Objective To study the expression of basic fibroblast growth factor (bFGF) mRNA in normal, ischemic and ischemic and reperfused skeletal muscles. Methods The model of hind limb ischemia-reperfusion injury in rats was used to detect the localization and expression of bFGF mRNA in normal and traumatic skeletal muscle by in situ hybridization and quantitative PCR. Results In the non-ischemic skeletal muscle, the bFGF mRNA was brown-black, dotted or dotted diffusely distributed in the cytoplasm, and the positive rate was 82%. The bFGF mRNA distribution did not change in skeletal muscle with ischemia, ischemia and reperfusion injury, but its staining intensity and density were significantly weakened. The positive rate of bFGF mRNA in muscle fiber was 52% and 22% respectively. Conclusions Traumatic injury leads to the decrease of endogenous bFGF mRNA expression in skeletal muscle, which leads to the decrease of bFGF production. It is one of the important reasons for the decrease of endogenous bFGF content in skeletal muscle during ischemia and reperfusion.