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建立不同产地藏药翼首草的UFLC指纹图谱分析方法。实验采用UFLC-PDA检测法,使用Agilent Proshell 120SB-C18色谱柱(4.6 mm×100 mm,2.7μm),以乙腈-0.2%磷酸水溶液为流动相梯度洗脱,流速1.0 mL·min-1,柱温为30℃,检测波长238 nm。建立了21个批次翼首草药材的共有图谱,提取了15个色谱峰作为指纹图谱共有峰,结合对照品对其中的5个色谱峰进行了指认,分别为绿原酸、马钱苷、獐芽菜苷、吴茱萸苷、大花双参苷A;21批药材中第13,18,20批次与对照指纹图谱比较相似度小于0.9,其他批次的相似度均大于0.9。该研究首次采用UFLC-PDA建立藏药翼首草的指纹图谱,该方法具有良好的精密度、稳定性和重复性,为翼首草质量控制和评价提供依据。
Establishment of UFLC fingerprinting method of Tibetan medicine Plectranta indica from different areas. UFLC-PDA was used to determine the mobile phase. The mobile phase was eluted with acetonitrile-0.2% phosphoric acid aqueous solution at a flow rate of 1.0 mL · min-1 using an Agilent Proshell 120SB-C18 column (4.6 mm × 100 mm, 2.7 μm) Temperature is 30 ℃, detection wavelength is 238 nm. The common map of 21 batches of Radix Zingiber officinale was established, 15 chromatographic peaks were extracted as the common peaks of fingerprint, and 5 of them were identified as chlorogenic acid, loganin, The similarity of the 13th, 18th and 20th batches of the 21 batches of Chinese medicinal herbs to the control fingerprints was less than 0.9, and the similarity of other batches were all above 0.9. In this study, the fingerprint of Tibetan medicine Zoysia was used for the first time using UFLC-PDA. The method has good precision, stability and repeatability, and provides the basis for the quality control and evaluation of Zoysia solani.