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目的:建立降脂宁片中绿原酸的含量测定方法。方法:采用高效液相色谱法测定降脂宁片中绿原酸的含量,色谱条件为色谱柱:Inertsil C18柱(4.6 mm×250 mm,5μm);流动相:乙腈-0.4%磷酸溶液(9∶91);检测波长:327 nm;进样量:10μL;柱温:30℃;流速:1 m L·min-1。结果:绿原酸质量在0.013 16~0.263 2μg范围内与峰面积具有良好的线性关系(r=0.999 9),平均加样回收率为98.26%(RSD=1.12%)。结论:该方法测定降脂宁片中绿原酸的含量,简便、准确,重复性好,可用于降脂宁片的质量控制方法。
Objective: To establish a method for determination of chlorogenic acid in Jiangzhining tablet. Methods: The content of chlorogenic acid in Jiangzhining Tablets was determined by HPLC. The chromatographic conditions were column: Inertsil C18 column (4.6 mm × 250 mm, 5 μm); mobile phase: acetonitrile-0.4% phosphoric acid solution (9 : 91); detection wavelength: 327 nm; injection volume: 10 μL; column temperature: 30 ° C; flow rate: 1 m L · min-1. Results: The linear range of chlorogenic acid was 0.013 16 ~ 0.263 2μg (r = 0.999 9). The average recovery was 98.26% (RSD = 1.12%). Conclusion: The method for the determination of the content of chlorogenic acid in Jiangzhining tablets is simple, accurate and reproducible. It can be used for the quality control of Jiangzhining tablet.