论文部分内容阅读
将分别携带抗番茄疮痂病菌Xanthomonas perforans T3小种基因Xv3、Rx4和Rx_(LA1589)的番茄材料Hawaii7981、PI128216和LA1589相互杂交,获得了包含535~1 655个单株的3个F2分离群体Hawaii7981×PI128216、Hawaii7981×LA1589和PI128216×LA1589。采用注射法对这3个F2群体的每个单株及亲本和感病对照OH88119进行抗T3小种鉴定,结果显示除OH88119外的所有单株对T3小种都具有过敏型抗性反应,表明Xv3、Rx4和Rx_(LA1589)为等位基因。根据Rx4基因的序列设计引物,从Hawaii7981、PI128216和LA1589中扩增该基因并测序,序列比对显示,来自于3个材料的Rx4位点编码区序列完全一致,表明这3个材料所携带的对番茄疮痂病菌T3小种的抗性由同一基因控制。
The Hawaii7981, PI128216 and LA1589 tomato materials carrying resistance genes Xv3, Rx4 and Rx_ (LA1589) of tomato Xanthomonas perforans T3 were crossed with each other to obtain three Hawaii7981 × F2 segregating populations containing 535 ~ 1 655 individuals PI128216, Hawaii7981 × LA1589 and PI128216 × LA1589. All the F2 populations and their parents and susceptible controls, OH88119, were tested for resistance to T3 by the injection method. The results showed that all the individuals except OH88119 had allergic resistance to T3 races, indicating that Xv3, Rx4 and Rx_ (LA1589) are alleles. The primers were designed according to the sequence of Rx4 gene. The gene was amplified from Hawaii7981, PI128216 and LA1589 and sequenced. The sequence alignment showed that the sequences of Rx4 sites from the three materials were completely identical, indicating that the three materials carried Resistance to the tomato scab pathogen T3 is controlled by the same gene.