血浆及尿中微量苯巴比妥、异戊巴比妥经提取后用薄层层析法分离,以氯化高汞—二苯偶氮碳酰肼甲醇溶液一次喷雾显色,较常用于巴比妥类的其他显色法灵敏度高,色点稳定,并呈明显浓度梯度(1～5μg梯度尤为显著),可在薄层上直接与苏巴比妥、异戊巴比妥标准对照比较其限量。本法操作简便,快速,结果可靠。 Plasma and urine trace phenobarbital, after the extraction of amylobalbitone by thin layer chromatography, with high chloride mercury - diphenyl azocine hydrazide methanol solution spray color, more commonly used in Pakistan Compared with other chromogenic methods, the sensitivity of colorimetric method is stable, the color point is stable, and the apparent concentration gradient (especially 1 ~ 5μg gradient) can be directly compared with the standard control of the pentobarbital and amobarbital on the thin layer Limited. This method is simple, fast and reliable.