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氯苯丁酯(安妥明,冠心平)是一种用于临床和理论研究的较新抗高血脂药物.本文报道一种灵敏、快速、准确的测定安妥明的分光光度法.其原理是安妥明在碱性介质中与盐酸羟胺反应生成4-氨苯氧基异丁氧肟酸,并与三价铁在酸性介质中形成紫色络合物,最大吸收波长为530nm.一般赋形剂对测定无干扰.其浓度在10～70μg/ml范围内符合比尔定律.氯苯丁酯浓度25μg/ml(在25ml内)与2M羟胺2～5ml及0.5M三氯化铁0.5～2.5ml反应,获得最大的颜色强度,最佳pH值为1.0～1.5,生成的有色物在室温2h内稳定.制备标准曲线:精密量取氯苯丁酯标准液(500mg/L)0.5～3.5ml,各置25ml容量瓶中,加碱性
Clofibrate (clofibrate, crown of peace) is a clinical and theoretical study of new anti-hyperlipidemic drugs.This paper reports a sensitive, rapid and accurate determination of clofibrate spectrophotometry. The principle is that the security Ming in alkaline medium with hydroxylamine hydrochloride to generate 4-aminophenoxy isobutyroximate and trivalent iron with a purple complex in acidic medium, the maximum absorption wavelength of 530nm.General excipients on the determination of Beer’s law is obeyed in the range of 10 ~ 70μg / ml.The reaction of chlorphenesin with 25μg / ml (in 25ml) with 2 ~ 5ml 2M hydroxylamine and 0.5 ~ 2.5ml 0.5M ferric chloride can be obtained by The maximum color intensity, the best pH value of 1.0 to 1.5, the resulting colored material stable at room temperature within 2h.Preparation of the standard curve: precise amount of clopidogrel standard solution (500mg / L) 0.5 ~ 3.5ml, each set 25ml Bottle, add alkaline