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本文介绍离体孵育条件下进行附睾管管腔灌流的技术。材料与方法一、插管灌流制备选用320～510克的Wistar大鼠,饲养在18～22℃,光照14小时,黑暗10小时,自由饮食。用尿酯(1克/公斤体重)麻醉后,取附睾组织,在冰浴条件下剔除附睾被膜,分离曲管叶,选好要插的管段,将拉细的聚乙烯塑料管(澳大利亚新南威尔斯Dural Eng & Pty公司)插入远端腔内,再用丝线(附睾尾用4/0,附睾体用7/0)结扎牢固,用内灌流液推出精浆,然后再做近端插管并用7/0线扎好,将此附睾段放入组织浴槽内(浴槽容积为20毫升),不断向浴槽内充气,用循环外水套控制温度在33±1℃,用每分钟一微升的灌流速度灌流附睾管管腔3小时。灌流
This article describes the technique of ex vivo perfusion of the epididymal duct. MATERIALS AND METHODS I. Preparation of intratracheal perfusion: 320-510 g Wistar rats were selected and housed at 18-22 ℃ for 14 hours under light and dark for 10 hours. After anesthesia with urethane (1 g / kg body weight), epididymal tissues were removed and epididymal capsular membranes were removed under ice-bath conditions. Tubular leaves were detached and the tube sections to be inserted were selected and the thinned polyethylene plastic tube (New South Australia Wells Dural Eng & Pty Company) into the distal cavity, and then silk (epididymal tail with 4/0, epididymal body with 7/0) ligation firm, with the introduction of seminal fluid seminal plasma, and then do the proximal plug Tube and tie 7/0 line, the epididymal segment into the tissue bath (bath volume of 20 ml), continue to inflate the bath, with a circulating water jacket to control the temperature at 33 ± 1 ℃, with a micro-minute Ascending perfusion perfusion epididymal canal 3 hours. Perfusion