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为了解决子囊霉素(1)发酵过程低溶氧的问题,本研究将透明颤菌(Vitreoscilla)血红蛋白基因(vgb)整合至1生产菌株吸水链霉菌(Streptomyces hygroscopicus)中,构建工程菌SIPI-FK520-2,通过RT-PCR分析检测到了vgb基因的转录表达,进一步利用CO结合差光谱分析也证实了工程菌中透明颤菌血红蛋白(VHb)具有生物活性。最后在发酵罐进行该工程菌的发酵培养研究,结果表明,与野生菌相比,工程菌发酵过程溶氧水平明显改善,1产量提高了38%,达到1 269.7 mg/L。
In order to solve the problem of low dissolved oxygen during the fermentation of ascomycin (1), Vitreoscilla hemoglobin gene (vgb) was integrated into a strain of Streptomyces hygroscopicus producing SIPI-FK520 -2, and the transcriptional expression of vgb gene was detected by RT-PCR analysis. Furthermore, the binding activity of Vitreoscilla hemoglobin (VHb) in the engineered bacteria was further confirmed by CO-coupled differential spectroscopy. Finally, the fermentation of the engineered bacteria was studied in a fermentor. The results showed that compared with the wild bacteria, the dissolved oxygen level of the engineered bacteria was significantly improved, and the yield of 1 was increased by 38% to reach 1 269.7 mg / L.