论文部分内容阅读
Inoculation of improved associative diazotrophs Klebsiella oxytoca NG 13 or Enterobacter cloacae E26 was performed in association with three va-rietes of rice ( Oryza sativa L.).namely Indica type C544,Japonica type T65 and Harebare,These improved diazotrophs carried a nifA containing plasmid pMC73A derived from Klebsiella pneumoniae M5al.In pot experiments the rice seedlings were planted in nonsterilized soil and grown under flooded conditions in the green house.By using 15N dilution technique it has shown that inoculation of these improved bacterial strains ( NGl3/pMC 73A or E26/pMC73A ) stimulated the growth rate and increased the nitrogen content of rice plants in comparison with that of Nif-phenotype K.o-xytoca NG1389 and E.cloacae E262.The nitrogen-fixing rate was 10-35% Ndfa after 120 days cultivation.
Inoculation of improved associative diazotrophs Klebsiella oxytoca NG 13 or Enterobacter cloacae E26 was performed in association with three va-rietes of rice (Oryza sativa L.). Both Indica type C544, Japonica type T65 and Harebare, These improved diazotrophs carried a nifA containing plasmid pMC73A derived from Klebsiella pneumoniae M5al. In pot experiments the rice seedlings were planted in nonsterilized soil and grown under flooded conditions in the green house. By using 15N dilution technique it has shown that inoculation of these improved bacterial strains (NGl3 / pMC73A or E26 / pMC73A) stimulated the growth rate and increased the nitrogen content of rice plants in comparison with that of Nif-phenotype Ko-xytoca NG1389 and E. cloacae E262. nitrogen-fixing rate was 10-35% Ndfa after 120 days cultivation.