论文部分内容阅读
目的观察孕酮对大鼠脑损伤后COX-2和IL-6表达及对神经功能的影响。方法雄性SD大鼠48只随机分为假手术组、脑损伤组和孕酮治疗组,按照改良的Feeney自由落体损伤装置制作大鼠脑损伤模型。孕酮治疗组伤后1、6 h腹腔注射孕酮16 mg/kg。各组于伤后24 h取材。用ELISA法检测COX-2和IL-6含量,用干湿重法测量脑组织含水量,用mNSS评分检测神经功能。结果孕酮治疗组与脑损伤组比较,COX-2和IL-6表达和脑水肿明显减少(P<0.05),神经功能明显改善(P<0.05)。结论孕酮可能通过降低COX-2和IL-6的表达,进而降低脑水肿,改善神经功能,发挥脑损伤保护作用。
Objective To observe the effect of progesterone on the expression of COX-2 and IL-6 and its neurological function after traumatic brain injury in rats. Methods Forty-eight male Sprague-Dawley rats were randomly divided into sham-operation group, brain injury group and progesterone-treated group. Brain injury models were made according to a modified Feeney free-fall injury device. Progesterone treatment group intraperitoneal injection of progesterone 1,6 mg / kg 1,6 h after injury. All groups were drawn 24 h after injury. The levels of COX-2 and IL-6 were measured by ELISA, the water content of brain tissue was measured by dry-wet weight method, and the neurological function was evaluated by mNSS score. Results Compared with the brain injury group, the expression of COX-2 and IL-6 in the progesterone group and the brain edema were significantly decreased (P <0.05) and the neurological function was significantly improved (P <0.05). Conclusion Progesterone may reduce brain edema, improve neurological function and protect brain injury through decreasing the expression of COX-2 and IL-6.