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目的 研究黏液型铜绿假单胞菌PA17中的mucA基因序列及其生物膜形成、生长速度和耐药性 ,并与铜绿假单胞菌标准菌株PAOI进行比较。方法 采用聚合酶链反应 (PCR)方法分别扩增PA17与非黏液型铜绿假单胞菌标准菌株PAOI的mucA基因 ,全自动荧光测序仪测序。同时用改良的平板培养法分别建立PA17和PAOI的生物膜模型 ,于 8h、2 4h、3d、6d用扫描电镜检测生物膜形成情况。稀释平板计数法比较PA17和PAOI的生长曲线 ,国际标准琼脂平皿二倍稀释法检测常用抗生素对上述两菌株的体外抗菌活性。结果 PA17mucA基因第 16 6 333位核苷酸缺失 ;其生物膜形成速度及生长速度明显慢于PAOI;PA17与PAOI对常用抗生素的耐药性一致。结论 发现一株含新的mucA突变基因的黏液型铜绿假单胞菌 ,其生物学特性不同于以往报道的黏液型铜绿假单胞菌 ,可能与mucA基因新的突变有关
Objective To study the sequence of mucA gene and its biofilm formation, growth rate and drug resistance in mucoid Pseudomonas aeruginosa PA17 and compare with the standard strain of Pseudomonas aeruginosa PAOI. Methods Polymerase chain reaction (PCR) method was used to amplify the mucA gene of PA17 and non-mucoid Pseudomonas aeruginosa standard strain PAOI respectively, and sequenced by automatic fluorescence sequencer. At the same time, the biofilm model of PA17 and PAOI was established by modified plate culture method, and the biofilm formation was detected by scanning electron microscopy at 8h, 24h, 3d and 6d respectively. The growth curve of PA17 and PAOI was compared with the dilution plate counting method. The antibacterial activity of common antibiotics against these two strains was tested by the international standard agar plate double dilution method. Results The nucleotide sequence of nucleotide 16 6 333 of PA17mucA gene was deleted. The biofilm formation rate and growth rate of PA17mucA gene were significantly slower than those of PAOI. PA17 and PAOI were consistent with common antibiotics. Conclusions A mucampoidal Pseudomonas aeruginosa strain containing the new mucA gene was found to be different from the previously reported mucoid Pseudomonas aeruginosa and may be related to a new mutation in the mucA gene