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棉铃虫核型多角体病毒 (HaNPV)分别与三氟氯氰菊酯、溴氰菊酯、氰戊菊酯、灭净菊酯、灭多威、辛硫磷、甲基对硫磷和乙酰甲胺磷等化学杀虫剂混合饲喂棉铃虫幼虫 ,统计致死中浓度LC50 ,计算增效比 ,测定虫体内与抗性有关的三种重要酶 :多功能氧化酶 (MFO)、羧酸酯酶 (CarE)、乙酰胆碱酯酶 (AChE)的活性。研究大豆卵磷脂对HaNPV致病性的影响。结果表明 :HaNPV与化学杀虫剂混合饲喂抗性棉铃虫 ,生测统计增效比均大于 1 0 ,特别是病毒与甲基对硫磷混用 ,增效比更是达到 3 53,表现出良好的增效作用。混剂感染抗性棉铃虫 ,虫体内MFO的活性比化学杀虫剂单用时降低 3～ 12倍 ,CarE和AChE的活性也比化学杀虫剂单用时低 ,HaNPV明显抑制了化学杀虫剂对MFO和CarE的诱导作用。HaNPV与大豆卵磷脂混用 ,提高了HaNPV对棉铃虫的感染致死率 ,缩短了致死中时间 (LT50 )。
Helicoverpa armigera nuclear polyhedrosis virus (HaNPV), respectively, with cyhalothrin, deltamethrin, fenvalerate, flumethrin, methomyl, phoxim, methyl parathion and acephate The chemical insecticides were mixed with the larvae of Helicoverpa armigera, the LC50 of lethal concentration was calculated, and the synergistic ratio was calculated to determine the three important enzymes related to resistance in the larvae: polyfunctional oxidase (MFO), carboxylesterase (CarE) , Acetylcholinesterase (AChE) activity. To study the influence of soybean lecithin on the pathogenicity of HaNPV. The results showed that both the HaNPV and the chemical insecticides were mixed with the resistant cotton bollworm, and the statistics of bioassay efficiency were all greater than 10, especially when the virus was mixed with methyl parathion, the synergistic ratio reached to 3 53 Good synergy. The activity of MFO was 3 to 12 times lower than that of chemical insecticide when mixed with H. armigera. The activity of CarE and AChE was also lower than that of chemical insecticide. HaNPV significantly inhibited the activity of chemical insecticide The induction of MFO and CarE. The combination of HaNPV and soybean lecithin increased the lethality of HaNPV to Helicoverpa armigera and shortened the lethal time (LT50).