论文部分内容阅读
本文综述了扩增10～40 kb大片段核酸的聚合酶链反应(LA-PCR)方法的原理、影响因素和条件优化,以及LA-PCR在扩增与克隆目的基因、研究基因组的多态性和分型等方面的应 用。
This review summarizes the principles, influencing factors and conditions of the polymerase chain reaction (LA-PCR) method for amplification of large fragments of 10-40 kb, as well as the amplification and cloning of genes of interest by LA-PCR and the genomic polymorphism And typing and other aspects of the application.