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离体条件下,研究了2个马铃薯品种在0~80 mmol NaCl胁迫下的试管苗生长、叶绿素含量、脯氨酸和丙二醛含量及氧化酶活性的反应.2品种苗高随盐浓度增加及培养时间的延长而显著降低.与品种紫花白相比,同一盐浓度下静石2号在苗鲜重、根长和根数等方面受抑制程度更强.盐胁迫培养3周后,试管苗叶片中叶绿素a,b与总量及类胡萝卜素含量在40~80 mmol高盐浓度下显著降低.两个品种试管苗中脯氨酸和丙二醛含量随盐浓度的增加而增加,而静石2号中脯氨酸和丙二醛的累积量和累积率都高于紫花白,表明在盐胁迫下静石2号膜脂过氧化程度相对较高.2品种SOD和POD活性均在低盐浓度(20 mmol)下显著增加而高盐浓度下降低.离体条件下,紫花白试管苗呈现出比静石2号高的SOD、POD活性以及较强的耐盐能力,表明其具有进一步在田间进行耐盐基因型筛选的必要性.
Under in vitro conditions, the responses of two potato cultivars to growth, chlorophyll content, proline and malondialdehyde content and oxidase activity under 0 ~ 80 mmol NaCl stress were studied.The seedling height of two varieties increased with the increase of salt concentration And cultured time significantly reduced.Compared with the varieties of purple white, under the same salt concentration, Shihshi No. 2 in the seedling weight, root length and root number and other aspects were more inhibited.Salt stress after three weeks of culture, The contents of chlorophyll a, b, total carotenoids and the content of carotenoids in the leaves were significantly decreased under the high salt concentration of 40 ~ 80 mmol. The content of proline and malondialdehyde in the two varieties of plantlets increased with the increase of salt concentration, The accumulative amount and accumulation rate of proline and malondialdehyde in Shi 2 were higher than that in purpurea, indicating that the lipid peroxidation of Jingshi No.2 was relatively high under salt stress.2 The activities of SOD and POD of the two cultivars were all lower Under salt concentration (20 mmol), the plantlets increased significantly under high salt concentration, but under high concentration of salt, the plantlets showed the higher activity of SOD and POD than that of Jingshi No.2, The need for salt-tolerant genotyping in the field.