Metastatic human HCC model is needed for the studies onmechanism and intervention of metastatic recurrence,Byusing orthotopic implantation of histologically intacttissues of 30 surgical specimens,a patient-likemetastatic model of human HCC in nude mice (LCI-D20)and a low metastatic model of human HCC in nude mice(LCI-D35) have been established.All mice withtransplanted LCI-D20 tumors exhibited extremely highmetastatic ability including spontaneous metastasis toliver,lungs,lymph nodes and peritoneal seeding.Remarkable difference was also found in expression ofsome of the invasiveness related genes and growthfactors between the LCI-D20 and LCI-D35 tumors.PAI-1increased gradually following tumor progression in LCI-D20 model,and correlated with tumor size and AFP level.Phasic expression of tissue intercellular adhesionmolecule-1 in this model was also observed.Using cornealmicropocket model,it was demonstrated that the vascularresponse induced by LCI-D20 tumor was stronger than thatinduced by LCI-D35 tumor.Similar report on metastatichuman HCC model in nude mice and human HCC cell linewith metastatic potential was rarely found in theliterature.This LCI-D20 model has been widely used forthe studies on intervention of metastasis,including anti-angiogenesis,antisense approach,metalloproteinaseinhibitor,differentiation inducer,etc.It is concluded thatthe establishment of metastatic human HCC model in nudemice and human HCC cell line with metastatic potentialwill provide important models for the in vivo and in vitrostudy of HCC invasiveness,angiogenesis as well asintervention of HCC recurrence. Metastatic human HCC model is needed for the studies on mechanism and intervention of metastatic recurrence, Byusing orthotopic implantation of histologically intact tons of 30 surgical specimens, a patient-like meta type of human HCC in nude mice (LCI-D20) and a low metastatic model of human HCC in nude mice (LCI-D35) have been established. All mice with transportedplanted LCI-D20 tumors exhibited extremely high metastatic ability including spontaneous metastasis toliver, lungs, lymph nodes and peritoneal seeding. Remarkable difference was also found in expression ofsome of the invasiveness related genes and growthfactorsbetween the LCI-D20 and LCI-D35 tumors. PAI-1increased gradually following tumor progression in LCI-D20 model, and correlated with tumor size and AFP level. Pascal expression of tissue intercellular adhesionmolecule- 1 in this model was also observed. Using cornealmicropocket model, it was demonstrated that the vascular response induced by LCI-D20 tumor was stronger than thatinduced by LCI-D35 tumor. similar report on metastatichuman HCC model in nude mice and human HCC cell line with metastatic potential was rarely found in the literature. This LCI-D20 model has been widely used forthe studies on intervention of metastasis, including anti-angiogenesis, antisense approach, metalloproteinase inhibitor, differentiation inducer, etc. It is said that that establishment of metastatic human HCC model in nudemice and human HCC cell line with metastatic potential wound provision important models for the in vivo and in vitrostudy of HCC invasiveness, angiogenesis as well as intervention of HCC recurrence .