目的：研究从卵巢上皮性癌（ＯＥＣ）患者肿瘤浸润淋巴细胞（ＴＩＬ）、肿瘤相关淋巴细胞（ＴＡＬ）及外周血淋巴细胞中分离扩增的γδＴ细胞对同种异体和自体肿瘤细胞的体外细胞毒作用，同时对αβΤ细胞的细胞毒活性也作了比较。方法：用抗体固相法扩增γδ／αβＴＩＬ，γδ／αβＴＡＬ及外周血γδ／αβＴ细胞；用密度梯度离心法或组织块培养法扩增原代ＯＥＣ细胞，并进行鉴定；用ＭＴＴ法测定γδ／αβＴ及外周血γδ／αβＴ细胞对同种异体／自体ＯＥＣ细胞的细胞毒活性。结果：体外扩增出的γδ／αβＴＩＬ，γδ／αβＴＡＬ及外周血γδ／αβＴ细胞对同种异体／自体ＯＥＣ细胞均具有较强且水平相近的细胞毒活性；扩增到７０％细胞纯度所需时间为：外用血γδΤ细胞（１４ｄ），γδＴＡＬ（２１ｄ）和γδＴＩＬ（２８ｄ）。结论：γδＴＡＬ有望成为ＯＥＣ过继细胞治疗的简便获得而有效的候选细胞。关 OBJECTIVE: To study the effect of γδT cells isolated from tumor infiltrating lymphocytes (TILs), tumor-associated lymphocytes (TALs) and peripheral blood lymphocytes in ovarian epithelial carcinoma (OEC) patients on in vitro cells of allogeneic and autologous tumor cells Toxic effects, while αβ T cells cytotoxic activity was also compared. Methods: The γδ / αβTIL, γδ / αβTAL and peripheral blood γδ / αβ T cells were amplified by antibody solid phase method. The primary OEC cells were expanded and purified by density gradient centrifugation or tissue culture. The expression of γδ / ΑβT and peripheral blood γδ / αβ T cells on allogeneic / autologous OEC cells cytotoxicity. RESULTS: The γδ / αβTIL, γδ / αβ TAL and γδ / αβ T cells expanded in vitro had strong and similar cytotoxic activity to allogeneic / autologous OEC cells. Time: external blood γδ T cells (14d), γδ TAL (21d) and γδ TlL (28d). Conclusion: γδTAL is expected to be a simple and effective candidate for OEC adoptive cell therapy. turn off