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[目的]该研究旨在研究云南蒙自的枇杷叶霉病病害,并为确定其有效的防治方法奠定基础。[方法]病原分离采用组织分离法,通过分生孢子悬浮液接种,用柯赫氏法则对其致病性进行验证。显微镜下观察菌丝形态及产孢结构并测量孢子大小,根据形态特征进行鉴定。[结果]在显微镜下发现分生孢子梗直立,顶端曲屈,深褐色,无隔膜,平滑,孢痕明显,(33.0~152.8)μm×(2.6~4.0)μm枝孢褐色或淡橄榄色,有胞痕,单胞或有1个隔膜,(7.1~19.0)μm×(1.9~5.9)μm分生孢子链生(2~4),椭圆形,偶球形,无孢痕,淡橄榄色,单胞,平滑,(2.1~9.4)μm×(1.2~2.6)μm。[结论]叶霉病在春梢和夏梢萌发期开始发病,秋梢发病最重,叶片正面和背面都长出烟煤状霉层,密布全叶,严重影响植株的光合作用,其病原菌初步鉴定为枇杷枝孢。
[Objective] The aim of the study was to study leaf blight disease of loquat in Yunnan Mengzi and to lay the foundation for its effective prevention and treatment. [Method] The pathogen was isolated by tissue isolation and inoculated by conidial suspension. The pathogenicity was verified by Koch’s rule. Morphology and sporulation were observed microscopically and the spore size was measured. The morphological characteristics were identified. [Result] The conidiophore was found under the microscope that the condyloma acuminatum was erect, the top was curved, dark brown, without membrane, smooth and obvious spore mark. The conidiophore was (33.0-152.8) μm × (2.6-4.0) There are cell lines, single cells or a diaphragm, (7.1 ~ 19.0) μm × (1.9 ~ 5.9) μm conidial chain (2-4), oval, even spherical, no spore marks, light olive, Monocytes, smooth, (2.1 ~ 9.4) μm × (1.2 ~ 2.6) μm. [Conclusion] The occurrence of leaf mold in the spring shoots and summer shoots began to develop. The shoots of autumn shoots were the heaviest. The leaf molds grew on the front and the back of the leaves. The whole leaf was densely covered, which seriously affected the photosynthesis of the plants. The pathogen was initially identified For loquat Cladosporium.