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本项研究选7只无自然感染HHBV的健康熊猴,实验感染HHBV。在这批熊猴出现血清学阳性的证据后,对其做肝活检(分早、中、晚期),经光、电镜动态病理学观察,并结合组化、免疫组化和分子原位杂交等法定位检测肝细胞内HHBV标志物。结果各猴肝脏组织病理学和超微病理学的动态变化与乙肝病人相似;并在猴肝细胞内定位检测出HBsAg、HBcAg和HBV-DNA复制,建立了理想的乙肝模型。
In this study, seven healthy bears who did not naturally infect HHBV were selected and infected with HHBV experimentally. Liver biopsy was performed in these bears after positive serological evidence (early, middle and late), dynamic pathological observation by light and electron microscope, combined with histochemistry, immunohistochemistry and molecular in situ hybridization Localization of HHBV markers in hepatocytes by the method of. Results The histopathological and ultrastructural changes of liver in each monkey were similar to those in hepatitis B patients. The replication of HBsAg, HBcAg and HBV-DNA were detected in monkey hepatocytes, and the ideal hepatitis B model was established.