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目的:探讨桂皮醛对急性髓系白血病细胞株U937的增殖和凋亡的影响及其相关机制。方法:以U937细胞为研究对象,CCK-8法测定细胞增殖活性;流式细胞术检测细胞周期、凋亡率、线粒体膜电位水平;ELISA法检测细胞上清液中VEGF浓度。结果:桂皮醛呈时间和剂量依赖性影响U937细胞生长。桂皮醛处理后U937细胞阻滞于G2/M期,细胞凋亡率明显增加,线粒体跨膜电位明显下降。ELISA检测表明,桂皮醛处理后U937细胞分泌VEGF水平明显下降。结论:桂皮醛抑制U937细胞增殖并诱导其凋亡。细胞增殖受抑与细胞周期受阻有关,线粒体介导的凋亡通路参与了桂皮醛诱导的凋亡。抑制U937细胞VEGF分泌可能是桂皮醛抗白血病重要机制之一。
Objective: To investigate the effects and mechanisms of cinnamic aldehyde on the proliferation and apoptosis of acute myeloid leukemia cell line U937. Methods: U937 cells were used as experimental materials. Cell proliferation was measured by CCK-8 assay. Cell cycle, apoptosis rate and mitochondrial membrane potential were measured by flow cytometry. The concentration of VEGF in supernatant was detected by ELISA. Results: Cinnamic aldehyde had a time-and dose-dependent effect on the growth of U937 cells. U937 cells arrested in G2 / M phase after cinnamic aldehyde treatment, apoptosis rate increased significantly, mitochondrial transmembrane potential decreased significantly. ELISA assay showed that the level of VEGF secreted by U937 cells after cinnamic aldehyde treatment decreased significantly. Conclusion: Cinnamaldehyde can inhibit the proliferation and induce the apoptosis of U937 cells. Inhibition of cell proliferation and cell cycle arrest, mitochondria-mediated apoptosis pathway involved in cinnamic aldehyde-induced apoptosis. Inhibition of VEGF secretion in U937 cells may be one of the important mechanisms of cinnamic aldehyde anti-leukemia.