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用SDSPAGEHPLC荧光检测方法研究了3例人血浆和红细胞蛋白或其亚基中的硒分布.在所采集的硒中毒病人,无病人和大骨节病人血浆中,分别检出5,5和4条含硒条带,在红细胞中则分别检出4,4和3条含硒条带.人血浆白蛋白或血浆硒蛋白P(SeP)和血红蛋白是人血硒的优势结合蛋白.另外,3个样品的血浆中517—434kDa部分条带可能是含硒蛋白,红细胞中分子量小于143kDa的可能是含硒肽分子.对这些条带的归属及其与地区硒水平之间的关系进行了讨论.
The distribution of selenium in 3 human plasma and erythrocyte proteins or their subunits was studied by SDSPAGE HPLC, and 5, 5 and 4 were detected in plasma of patients without selenium and patients with serous hyperplasia Selenium bands were detected in red blood cells were detected in 4 and 3 selenium bands.Human plasma albumin or plasma selenoprotein P (SeP) and hemoglobin is the dominant binding protein of human blood selenium.In addition, three samples Of the 517-434 kDa portion of the plasma may be selenoprotein, the molecular weight of less than 143 kDa in red blood cells may be selenium-containing peptide molecules.The relationship between the ownership of these bands and their regional selenium levels is discussed.