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【目的】研究斜纹夜蛾Spodoptera litura幼虫感染莱氏野村菌Nomuraea rileyi后的抗氧化防御机制。【方法】通过测定斜纹夜蛾各龄幼虫超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)等的活性,探讨染菌后不同侵染阶段,幼虫抗氧化酶活性的变化趋势和不同接种方法对抗氧化酶活性的影响。【结果】在斜纹夜蛾染菌幼虫和未接种幼虫(对照)中均未测出POD活性;各龄幼虫在接种莱氏野村菌后SOD和CAT活性显著高于对照,但随着侵染过程的进行,2-3龄幼虫的SOD和CAT活性在16 h左右达峰值后迅速降低,而4-5龄幼虫SOD和CAT活性自染菌初期增强后,变化较平缓,至60-72 h后才低于对照。喷雾法与浸蘸法接种后,各龄幼虫SOD和CAT活性显著高于对照,且对于2-3龄幼虫,浸蘸法对抗氧化酶活性的影响显著高于喷雾法,而对于4-5龄幼虫而言两处理方式之间活性差异不显著。【结论】斜纹夜蛾感染莱氏野村菌后,其体内抗氧化酶活性变化很大,SOD和CAT活性先升后降,且其变化趋势与幼虫所处的发育阶段密切相关。在体壁接种量相当的情况下,浸蘸法对2-3龄幼虫SOD和CAT活性的影响大于4-5龄幼虫。
【Objective】 To investigate the antioxidant defense mechanism of Spodoptera litura larvae infected with Nomuraea rileyi. 【Method】 The activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) in different instar larvae of Spodoptera litura were determined. The larval resistance Changes of Oxidase Activity and Effects of Different Inoculation Methods on Antioxidant Enzymes Activity. 【Result】 The activity of POD was not detected in S. litura larvae and non-inoculated larvae (control). The activities of SOD and CAT in different instar larvae were significantly higher than those in the control The SOD and CAT activities of 2-3 instar larvae decreased rapidly at about 16 h, while the activity of SOD and CAT at 4-5 instar larvae increased gradually from the initial stage after the infection. After 60-72 h Only lower than the control. The activity of SOD and CAT in larvae of each instar larvae were significantly higher than that of the control after inoculation by spraying and dipping method, and the dipping method had significantly higher antioxidant enzyme activity than the spraying method for 2-3 instar larvae, There was no significant difference in activity between the two treatments in larvae. 【Conclusion】 The infection of Spodoptera litura by the Spodoptera litura showed that the activity of antioxidant enzymes varied greatly in vivo and the activities of SOD and CAT increased first and then decreased, and the trends were closely related to the developmental stage of larvae. In the case of the same volume of wall inoculation, the dipping method had more effect on the activities of SOD and CAT than the 2-3 instar larvae.