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The polymorphism of mitochondrial DNA (mtDNA) D-loop of 83 individuals from nine Chinese indigenous sheep breeds and two imported sheep breeds was studied with five endonucleases,Hinf I,Msp I,Sau3A I,Xsp I and Taq I,using PCR-RFLP.The results indicated that there existed two basic haplotypes in the region ofmtDNA D-loop.It couldbe inferred that Chinese indigenous sheep breeds originated from two mateal ancestors.The average polymorphic degree (π value=0.0421%) of mtDNA D-loop showed that the genetic diversity of mtDNA of Chinese indigenous sheep breeds was very low.