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以国产柴油机排气口废气采集物为受试物,用噻唑蓝(MTT)染色的方法检验受试物各浓度作用12 h、24 h、36 h的细胞相对存活率;单细胞凝胶电泳技术检测作用2 h、4 h、8 h受试物致细胞的DNA损伤。随着染毒时间的延长和染毒剂量的增加,细胞存活率下降;柴油机废气颗粒物引起细胞的拖尾率、尾矩和细胞尾部DNA含量增加。柴油机废气颗粒物的各浓度组在不同时间诱导的细胞拖尾率和尾部DNA含量均高于阴性对照组,差异有统计学意义(P<0.05)。提示柴油机废气颗粒物可以降低细胞的相对存活率,并不同程度地致NIH/3T3细胞的DNA损伤。
The exhaust gas collection from domestic diesel engine exhaust was used as the test substance, and the relative cell viability at 12, 24 and 36 h of each concentration of test substance was tested by MTT staining. The single cell gel electrophoresis Detection of DNA damage caused by the test substance 2h, 4h, 8h. With the prolongation of exposure time and the increase of exposure dose, cell survival rate decreased; particulate matter of diesel engine caused cell tailing rate, tail moment and DNA content in tail cell increased. The tailing percentage and tail DNA content of diesel particulate matter in each concentration group at different times were higher than those in the negative control group, the difference was statistically significant (P <0.05). It is suggested that diesel particulate matter can reduce the relative survival rate of cells, and to some extent, DNA damage of NIH / 3T3 cells.