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目的研究山西省艾滋病病毒Ⅰ型(HIV-1)主要流行株env基因gp120区变异特性及其分子流行病学的意义。方法应用套式聚合酶链反应(Nested-PCR)对山西省56份HIV-1毒株的gp120区进行扩增,使用ABI测序仪测序,应用BLAST、GCG和MEGA等序列分析软件,对gp120区序列进行分析。结果山西省HIV-1主要流行株在系统进化树上与国际参考株B.CN.RL42-U71182汇聚在一起。山西省56份HIV-1毒株gp120区的共享序列与同源性较高的国际参考株B.CN.RL42-U71182相比,N-糖基化位点增加了5个,并在V1-V5、C2、C3、C5区段存在独特的特征性氨基酸位点。V3顶端四肽存在着9种类型:GPGQ(39.29%)、GPGR(19.64%)、GPGK(17.86%)、GQGR(10.71%)、GLGR(5.36%),GQGQ、GPGA、RLRR与RPRK分别为1.79%。结论在山西省HIV感染者中,HIV-1主要流行株仍为B’亚型,其HIV感染者中流行毒株的基因变化较大,gp120基因N-糖基化位点的增加和特征性氨基酸的存在,以及多种V3顶端四肽的存在,表明在较长的流行时间里已经有形成自己独特的序列特征模式的倾向。
Objective To investigate the variation of env gene gp120 in HIV-1 major epidemic strains of Shanxi Province and its molecular epidemiological significance. Methods Nested-PCR was used to amplify the gp120 region of 56 HIV-1 strains in Shanxi Province. Sequencing was performed by ABI sequencer. Sequence analysis software such as BLAST, GCG and MEGA was used to amplify the gp120 region of gp120 Sequence analysis. Results The main epidemic strains of HIV-1 in Shanxi province were clustered together with the international reference strain B.CN.RL42-U71182 on the phylogenetic tree. The shared sequences of the gp120 region of 56 HIV-1 strains in Shanxi Province showed 5 more N-glycosylation sites compared with the international reference strain B.CN.RL42-U71182 with higher homology, V5, C2, C3, C5 segments exist unique characteristic amino acid sites. There are 9 types of V3 top tetrapeptide: GPGQ (39.29%), GPGR (19.64%), GPGK (17.86%), GQGR (10.71%), GLGR (5.36%), GQGQ, GPGA, RLRR and RPRK were 1.79 %. Conclusions Among the HIV-infected people in Shanxi province, the major HIV-1 strains are still B ’subtype, and the gene mutations of the epidemic strains in HIV-infected persons are large. The increase and characteristic of the N-glycosylation site of gp120 gene The presence of amino acids, as well as the presence of a variety of V3-top tetrapeptides, suggests that there has been a tendency to form its own unique sequence signature pattern over a longer prevailing time.