以２组核背景相同的玉米正常杂种、不育杂种和恢复杂种为材料，分３期播种鉴定育性。同时采用双垂直板聚丙烯酰胺凝胶电泳的方法，测定小孢子处于单核早期，二核早期和散粉前花药中的脂酶，过氧化物酶和细胞色素氧化酶同工酶酶谱。结果指出：玉米中强恢复基因Ｒｆ４能完全恢复Ｃ型和Ｙ＿（Ⅱ－１）型不育系的育性，恢复性较稳定，不易受温度影响。在小孢子发育的３个时期，不育杂种花药中的３种同工酶酶谱与正常杂种差异很大，而恢复杂种的酶谱基本上同正常杂种，仅有个别谱带与不育杂种相似。Ｃ型和Ｙ（Ⅱ－１）型不育胞质在脂酶和过氧化物酶酶谱上的表现完全一致，在细胞色素氧化酶上却有些不同 Two groups of normal corn with the same nuclear background, sterile hybrids and restoring hybrids were used as material to sow in three stages to identify fertility. At the same time, the double-vertical plate polyacrylamide gel electrophoresis method was used to determine the enzyme activity of lipase, peroxidase and cytochrome oxidase isozymes in early mononuclear, early nucleus and anther before anther. The results showed that the strong maize restorer gene Rf4 could completely restore the fertility of C-type and Y-type Ⅱ-1 CMS lines, and the recovery was stable and not easily affected by temperature. In the three stages of microspore development, the three isoenzyme patterns in sterile anthers differed significantly from those in normal hybrids, but the restoring hybrids were basically the same as the normal hybrids, with only individual bands and sterile hybrids similar. C and Y (Ⅱ-1) type sterile cytoplasm in the lipase and peroxidase zymograms on the performance of exactly the same, but a little different in cytochrome oxidase