目的建立大细胞肺癌免疫组化试剂盒并在临床初步应用。方法基于所制备的抗大细胞肺癌单抗和HRP酶标兔抗大细胞肺癌单抗抗体,筛选出大细胞肺癌免疫组化试剂盒最优检测方法,并对其性能进行了鉴定和确证。结果大细胞肺癌免疫组化试剂盒能对大细胞肺癌进行特异性鉴定,试剂盒在4℃条件下保存18个月而质量不变,具有良好的批内、批间重复性和良好的稳定性。结论本研究成功研制出了敏感、特异、稳定的大细胞肺癌免疫组化试剂盒并能在临床初步应用。 Objective To establish a large cell lung cancer immunohistochemical kit and its clinical application. Methods Based on the prepared monoclonal antibodies against large cell lung cancer and HRP-labeled rabbit anti-large cell lung cancer monoclonal antibodies, the optimal detection method for large cell lung cancer immunohistochemical kits was screened out, and its performance was identified and confirmed. Results The large cell lung cancer immunohistochemistry kit could specifically identify large cell lung cancer. The kit was stored at 4 ℃ for 18 months without change in quality, with good intra-assay, inter-assay reproducibility and good stability . Conclusion This study successfully developed a sensitive, specific and stable large cell lung cancer immunohistochemistry kit and its clinical application.