目的研究As2O3对人淋巴瘤Raji细胞株的影响,探讨其可能的作用机制.方法采用改良MTT方法观察As2O3对Raji细胞的增殖抑制效应;采用半定量RT-PCR方法检测As2O3对Raji细胞VEGF121和VEGF165MRNA表达的影响.结果As2O3对Raji细胞表现增殖抑制效应,效应呈时间、剂量依赖性,建立回归方程为:抑制率(IR)=0.531Dose+0.481Time(P<0.05),半数抑制度(IC50)为(3.84+1.41)μM.半定量RT-PCR方法检测Raji细胞同时表达VEGF121和VEGF165两种剪接变异体,二者的表达 Objective To study the effect of As2O3 on Raji cell line of human lymphoma and to explore its possible mechanism.Methods The inhibitory effect of As2O3 on proliferation of Raji cells was observed by modified MTT method.The expression of VEGF121 and VEGF165 mRNA in Raji cells was detected by semi- (IR) = 0.531Dose + 0.481Time (P <0.05). The IC50 value of As2O3 in Raji cells was significantly higher than that in control group (P <0.05) .Conclusion As2O3 can inhibit proliferation of Raji cells in a time and dose- (3.84 + 1.41) μM. Semi-quantitative RT-PCR was used to detect the expression of both VEGF121 and VEGF165 splicing variants in Raji cells